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GitHub - joybio/multiPrime: multiPrime is a mismatch-tolerant minimal primer set design tool for large and diverse sequences (e.g. Virus). Here is a web-based version (test: http://multiPrime.cn))
Sequence Alignment Tool | Benchling
Primer Design | Geneious
Learn - Unipro UGENE
Multiple sequence alignment - Wikipedia
CLC Manuals - clcsupport.com
New specific primers for amplification of the Internal Transcribed Spacer region in Clitellata (Annelida) - Liu - 2017 - Ecology and Evolution - Wiley Online Library
How do I design degenerate primers based on an alignment? – Geneious
Primer Design | Geneious
How to align designed PCR FW & REV Primers with DNA sequence? Which software is best to use? | ResearchGate
How to design Primers for PCR
Guide to Using the Multiple Sequence Alignment Viewer
Multiple-Sequence Alignment | DNA Sequencing Software - Sequencher from Gene Codes Corporation
AlleleID: Real-Time PCR Assay Design Software for Pathogen Detection and Bacterial Identification
FastPCR manual
Guide to Using the Multiple Sequence Alignment Viewer
Sequence alignments and validation of PCR primers used to detect phylogenetically diverse nrfA genes associated with dissimilatory nitrate reduction to ammonium (DNRA) - ScienceDirect
Multiple sequence alignment - Wikipedia
A tool to automatically design multiplex PCR primer pairs for specific targets using diverse templates | Scientific Reports
Sequence Data module | BIONUMERICS
Multiple alignment of primers designed through Primer-BLAST tool with... | Download Scientific Diagram
Primer-BLAST now designs primers for a group of related sequences - NCBI Insights
FastPCR manual
Primer Design | Geneious
Molecular Biology Freeware for Windows
PCR primer design, in silico PCR and oligonucleotides